SARS CoV 2 - iClean

SARS-CoV-2 (COVID-19 Virus) Research - Study 1

Virucidal Hard-Surface Efficacy Test - Severe Acute Respiratory Syndrome-related Coronavirus 2 (SARS-CoV-2) (COVID-19 Virus).
Microbe(s): SARS-CoV-2, COVID 19


ABSTRACT
EcoloxTech systems were used to generate a 130 ppm electrolyzed water solution of hypochlorous acid. A greater than 4-log reduction or complete kill was obtained on all 3 replicates studied after a 5 minute contact time. This test was performed in order to substantiate virucidal efficacy claims for a test substance to be labeled as a virucide by determining the potential of the test substance to disinfect hard surfaces contaminated with Severe Acute Respiratory Syndrome-related Coronavirus 2 (S...

Disinfecting against SARS-CoV-2 (Coronavirus) using HOCl

Hypochlorous acid may be the disinfectant of choice for coronaviruses.

COVID-19 (Coronavirus Disease 2019) Virus Structure and Mechanism of Infection

Coronavirus Disease 2019 (COVID-19) is a novel virus. It causes severe acute respiratory syndrome. Severe acute respiratory syndrome coronavirus 2 (SARSCoV-2) is the agent responsible for a surface-to surface communicable disease that had infected
approximately 64.3 million persons as of December, 2020. Health care providers need options to limit and control the spread of the virus between themselves and patients. COVID-19 is an enveloped, positive-sense, singlestranded RNA virus approximately 60 to 140 nm in diameter. The virus’s Spike glycoprotein S1 firmly binds to the angiotensin converting enzyme 2 (ACE2) receptor, which allows entry into the host cell.2-4 COVID-19 infection creates a cytokine storm, severe pneumonia, multiple-organ failure, and acute cardiac injury. Transmission occurs through touch or aerosol spreading of the virus. A common pathway of spreading this virus is through respiratory aerosols from an infected person.7 During speech, humans emit thousands of oral fluid droplets per second that can remain airborne for 8 to 14 minutes.8 COVID-19 is detectable for up to 3 hours in surface aerosols, for up to 4 hours on copper, for up to 24 hours on cardboard, and for up to 2 to 3 days on plastic and stainless steel.9,10 There is a need to disinfect surfaces potentially exposed to COVID-19 to prevent transmission.

Use of Disinfectants

On contact with the virus, a disinfectant agent changes the protective protein coat, which loses its structure and aggregates, forming clumps of proteins with other viruses.9,10 Currently, the US Environmental Protection Agency has recommended
numerous disinfectants against COVID-19 including hypochlorous acid (HOCl).11 The mechanism of disinfection involves the destroying of the cell wall of microbes or viruses, allowing the disinfectant to destroy or inactivate them.12-27 This article focuses on HOCl.

Hypochlorous Acid

An ideal disinfectant and sanitizer must be nontoxic to surface contact, noncorrosive, effective in various forms, and relatively inexpensive. HOCl may be the disinfectant of choice for coronaviruses in an oralmaxillofacial surgery (OMS) office.
HOCl is an endogenous substance in all mammals and is effective against a broad range of microorganisms. Neutrophils, eosinophils, mononuclear phagocytes, and B lymphocytes produce HOCl in response to injury and infection through the mitochondrial membrane–bound enzyme known as ‘‘respiratory burst nicotinamide adenine dinucleotide phosphate oxidase.’’28 HOCl selectively binds with the unsaturated lipid layer and subsequently disrupts cellular integrity. Between pH levels of 3 and 6, the predominant species is HOCl that has maximal antimicrobial properties.29,30 HOCl is a powerful oxidizing agent. In aqueous solution, it dissociates into H+ and OCl–, denaturing and aggregating proteins.30 HOCl also destroys viruses by
chlorination by forming chloramines and nitrogencentered radicals, resulting in single- as well as double-stranded DNA breaks, rendering the nucleic acid useless and the virus harmless.31


HOW TO MAKE HOCl

The traditional method of manufacturing HOCl requires specialised equipment to electrically generate it from a chemical solution. HOCl can now be made by simply dissolving an Atom Plus tablet into water accoring to the follwoing tabel: